Abstract
Studies on amino acid incorporation into isolated mitochondria in vitro were carried out in normal and hypertrophic hearts obtained after aortic constriction. The incorporation of labeled amino acids in vitro was triggered immediately after 6 h of operation as an early response to the induced work load and at further time periods the incorporation increased rather slowly and reached a maximum at 8 days after aortic constriction. At a further time period when the hypertrophy developed to an advanced stage, the incorporation was drastically reduced. The cytosol obtained from normal and hypertrophic hearts had a stimulatory effect on the labeled amino acid incorporation in vitro into isolated mitochondria from normal hearts. This stimulatory effect was maximum with cytosol obtained from 40% hypertrophic animals. Partial characterization of the factors involved in the stimulation of amino acid incorporation in vitro revealed the involvement of heat-labile, nondialyzable, and ammonium sulfate-precipitable protein stimulatory factors.
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