Abstract
In order to determine the most suitable cytokinin for the micropropagation of carob (Ceratonia siliqua L.), the effect of four cytokinins: BAP, zeatin, kinetin and 2-iP, was tested on explants derived from young seedlings of seven days. Organogenesis is better in the presence of BAP (0.5 mg/l), while buds growth is favored by zeatin (0.5 mg/l). The combination of the most suitable cytokinin (BAP at 0.5 mg/l) with IBA improves the caulogenesis when the concentration of this latter is low (0.1 mg/l); high concentrations of IBA have an inhibitory effect on elongation and neoformation of shoots and leaves. The multiplication and growth of shoots are more favorable on WPM medium in the presence of BAP (0.5 and 1 mg/l) alone or combined with 0.5 mg/l with GA3, while rooting is mainly favored by IBA, especially at 2 mg/l.
Highlights
This Carob (Ceratonia siliqua L.) is a Fabaceae Caesalpinioideae characteristic of Mediterranean regions and more or less equivalent climates
In order to determine the most suitable cytokinin for the micropropagation of carob (Ceratonia siliqua L.), the effect of four cytokinins: BAP, zeatin, kinetin and 2-iP, was tested on explants derived from young seedlings of seven days
The combination of BAP at moderate concentrations with low concentrations of GA3 has been used successfully [28] [32] [33] during shoot multiplication phase from various carob explants, while we reported negative effect of this combination on shoots multiplication from cotyledonary buds [29]
Summary
This Carob (Ceratonia siliqua L.) is a Fabaceae Caesalpinioideae characteristic of Mediterranean regions and more or less equivalent climates Spontaneous, it is currently cultivated on a large scale for its ecological and economic virtues. In Morocco, except of the arid zones, this species is present everywhere, in the same bioclimatic stage as Thuja, Red Juniper, Pistachio tree as well as in the upper part of argan tree. It is more abundant in the Middle Atlas (Khenifra, especially the region of Oued Srou), as well as in the south where it often mixes with Thuya and Phenicia juniper [6]. It is found in Sais, Anti-Atlas, Northern High Atlas, Central Plateau, Pre-Rif and Western Rif, especially in the region of Chefchaouen, both in the spontaneous and domesticated state, as in Mokrisset Circle [7] [8] [9] [10]
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