Abstract
The effect of two cytokinins (BAP, m-Topolin) tested singly or in combination with a mixture of amino acids (glycine, glutamine, cysteine) on multiplication rate and quality of <em>Pelargonium</em> x <em>hederaefolium</em> and <em>Pelargonium</em> x <em>hortorum</em> shoots were investigated. For both <em>Pelargonium</em> cultivars, the application of cytokinins greatly increased the production of axillary and adventitious shoots compared with the shoots produced on the medium without growth regulators. In the case of <em>P</em>. x <em>hederaefolium</em> no significant differences it were found in the number of shoot formed for BAP and m-Topolin treatments. For <em>P</em>. x <em>hortorum</em> the most effective multiplication of shoots was obtained on the medium supplemented with m-Topolin (1.0 mg l<sup>-1</sup>). Moreover, in the presence of m-Topolin an improvement of shoot quality of both genotypes has been observed (well developed shoots with soft green leaves). It was particularly apparent for <em>P</em>. x <em>hortorum</em> shoots. The addition of amino acids to a cytokinin medium increased slightly the number of formed shoots of <em>P</em>. x <em>hederaefolium</em> and influenced the shoot quality of both <em>Pelargonium cultivars</em> (well developed leaf blades, uniform growth of shoots in clumps, slower process of shoot senescence).
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