Abstract
Cyclophilin (Cyp) is peptidyl–prolyl isomerase (PPIase), and it has many biological functions, including immune response regulation, antioxidants, etc. Cyp from red algae is known for its antioxidant and antifungal activity. However, the other biological effects of Cyp from Pyropia yezoensis are unclear. In this study, we synthesized Cyp from P. yezoensis (pyCyp) and examined its biological activity on IEC-6 cells. First, the MTS assay showed that pyCyp increased cell proliferation in a dose-dependent manner. pyCyp activated the EGFR signaling pathway that regulates cell growth, proliferation, and survival. It induced intracellular signaling pathways, including the Ras signaling pathway. In addition, we observed cell cycle-related proteins. pyCyp increased the expression of cyclin A, cyclin E, and Cdk2, and decreased the expression of p27 and p21 proteins. These results indicate that pyCyp stimulates cell proliferation via the EGFR signaling pathway and promotes cell cycle progression in intestinal epithelial cells. Therefore, we suggest pyCyp as a potential material to promote the proliferation of intestinal epithelial cells.
Highlights
Pyropia yezoensis (P. yezoensis) has been cultivated and consumed as food primarily in China, Japan, and Korea [1]
We investigated the effect of the physiological activity of pyCyp in intestinal epithelial cells (IEC)-6 cells, which is a normal small intestinal epithelial cell derived from rat
The results indicate mean ± S.D in IEC-6 cells were seeded in 48-well plates at 2 × 104 cells/well with medium supplemented with triplicate. * Effect p < 0.05 versus the corresponding control group and * p < 0.05 used to define statistical pyCyp Treatment on epidermal growth factor receptor (EGFR)
Summary
Pyropia yezoensis (P. yezoensis) has been cultivated and consumed as food primarily in China, Japan, and Korea [1]. We used Cyp from P. yezoensis (pyCyp) to identify the precise factors involved in small intestinal epithelial cell proliferation. We investigated the effect of the physiological activity of pyCyp in IEC-6 cells, which is a normal small intestinal epithelial cell derived from rat. We observed their mechanisms of promotional cell proliferation via the activation of EGF and EGF signal pathway proteins when pyCyp was treated in IEC-6 cells. Based on these results, we intend to present the possibility of using the derived protein from Pyropia yezoensis as a gastrointestinal protection material
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