Effect of cyanobacterial and microalgal biomass on anther culture response of wheat (Triticum aestivum L.)

Abstract

In the present work the effect of microalgal and cyanobacterial biomass on anther cultures of wheat (Triticum aestivum L.) was studied. Investigations were made on the influence of media supplemented with a biomass of four terrestrial and fresh-water microalgal and cyanobacterial strains (1-2 g l-1) on the androgenic response, frequency of microspore-derived embryo-like structures, and their regeneration capacity in anther cultures of wheat. The addition of 1 g L-1 of cyanobacterium biomass MACC 643 to the induction and regeneration media significantly improved the androgenic response by 50% in the genotype Mv Pálma, and reduced the requirement for the synthetic auxin 2,4-D.