Abstract
Cynomolgus monkey oocytes were recovered from healthy or atretic follicles greater than 1000 micron in diameter at day 8 of gonadotrophin stimulated cycles and cultured in vitro, cumulus enclosed or cumulus free, for 2 days. Germinal vesicle breakdown (GVBD) occurred in 26.7% of healthy cumulus enclosed oocytes (n = 60) compared to 52.6% of atretic cumulus enclosed oocytes (n = 38). The mechanical removal of the cumulus cell mass increased the GVBD rate of the healthy oocytes (56.5%, n = 23) and accelerated the passage of the atretic oocytes (n = 23) from metaphase I (4.3%) to metaphase II (47.8%). In the healthy primate follicle, the dictyate stage could be maintained by an inhibitory substance secreted by the cumulus; follicular atresia could either decrease the synthesis of this substance and/or induce the metabolism of a stimulatory substance.
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