Abstract
The study is aimed at developing appropriate media for the mantle explant culture of abalone Haliotis varia. The effect of different media viz., L-15, Ham's F12, M199 was studied in combination with 10% Fetal Calf Serum (FCS) and 10% tissue extracts of gonad, mantle and whole body of abalone, H. varia to understand cell behaviour, cell yield and cell adherence in mantle explant culture of H. varia. Cultures with L-15 media gave better cell yield and M199 promoted better cell adherence. Addition of mantle extract to all media enhanced the cell yield to a maximum followed by whole body extract. Addition of whole body extract facilitated in cell adherence followed by the addition of mantle extract.
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