Abstract
Superoxide dismutase (SOD) was produced by the fungal strain Humicola lutea 110. Some of the factors influencing both enzyme and biomass production were studied in shake-flask cultures: glucose and nitrogen concentration, inoculum quantily, and temperature. The best results were obtained for a fermentation performed with 4% glucose, 0.152% nitrogen source, and 8% inoculum at 30°C. The time course of SOD biosynthesis showed two maxima (84.1 and 120.8 U/mg protein SOD activity respectively), which correspond to the maxima of the biomass. The dramatic increase in SOD activity during the late stationary stage indicated that the processes of O 2 − generation were intensified in the cells. Mn-SOD was responsible for the modulation of total SOD activity at both maxima.
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