Abstract
This experiment amid to study the effect of cultivars, growth regulator and number of sub-cultures on the shoots formation of strawberry plantlets during multiplication stage and Random amplified polymorphic DNA (RAPD) analysis of strawberry cultures in vitro during the fourth sub-culture. This experiment included 40 treatments, which were the combination between two strawberry cultivars (Festival and Sweet Charlie), five treatments of growth regulator (BA and GA3) and four number of sub-cultures during shoots formation (multiplication stage). The obtained results showed that, the maximum increment of growth measurement of strawberry plantlets were recorded by Sweet Charlie cultivar .In addition , using ½ MS-medium without supplemented with any growth regulators (BA and GA3) being the superior treatment for increasing both number of leaves per shoot and shoot length. On the other hand, generally, the fourth sub-culture being the most effective treatment on the growth measurement of strawberry plantlets during multiplication stage. Furthermore, Random amplified polymorphic DNA (RAPD) analysis varied according to the two tested cultivars and the type of for production of disease resistant plants and in plant breeding and crop improvement programs (Mohamed, 2003).
Highlights
Plant growth regulators (PGRs) are organic compounds naturally occurring in plants, which regulate many processes in plant development
Between days 2 and 8, Gibberellic acid3 (GA3) concentrations in corms treated with T.harzianum or A. migulanus, followed by inoculation with F. oxysporum f. sp. gladioli were significantly higher than in corms treated with F. oxysporum f. sp. gladioli alone, or in control corms (P< 0.001)
The GA3 concentrations in corms treated with A. migulanus followed by inoculation with F. oxysporum f. sp. gladioli, were significantly lower than in those inoculated with A. migulanus alone (P>0.05)
Summary
Plant growth regulators (PGRs) are organic compounds naturally occurring in plants, which regulate many processes in plant development. The combination between T. harzianum and A. migulanus was prepared by mixing the same volume of suspension of antagonist in beaker, immersing surface sterilized corms in the mixed suspension for 30 min and inoculating with the pathogen, as described above. Between days 2 and 8, GA3 concentrations in corms treated with T.harzianum or A. migulanus, followed by inoculation with F. oxysporum f.
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
