Effect of Cu(II) on Shoot Regeneration In triticum Aestivum Tissue Cultures Probed by the Fast Fluorescence (Jip-Test)

Abstract

Copper concentrations (0.1, 1, and 100 µM) significantly enhance shoot regeneration from Triticum aestivum L. calli and stimulate root formation. The results of the JIP-test show that though the phenomenological activities for trapping and electron transport decrease by increasing [Cu], the corresponding specific activities increase. Thus, though a fraction of PSII reaction centers (RCs) become inactivated, the rest of the RCs become more active when [Cu] in the culture medium increases. We recognize an optimization strategy that appears to regulate at low [Cu] a high photosynthetic activity per tissue, and at high [Cu] a high specific photosynthetic activity, in parallel to the stimulation of shoot formation.