Abstract
Aim: The aim of the study was to investigate the histological changes of rat epididymis with the effect of cryopreservation on 15, 30, 45 and 60 days freezingthawing. Methods: The study was performed on male rats, epididymides collected were immediately subjected to 15, 30, 45 and 60 days freezing process, after completion of preservation time they were thawed at 37°C for 10’ were fixed in formalin for histological investigation. Results: After15, 30, 45 and 60 days of cryopreservation rat epididymis showed reduce in number of sperm in lumen, degenerative changes in tissue shows elongated tubules and loss of epithelium and connective tissue. Conclusion: In conclusion, the histological changes obtained after freezing storage were reasonable; however, the cryopreserved tubules sheets could not maintain their original layered structure. This work needs to be done to better preservation of the rat epididymis.
Highlights
Cryopreservation of reproductive cells and tissues has become an increasingly important methodology for future fertility advantages [1,2]
Scientist regards to cryopreservation be crucial for enabling technology to the progression from preclinical and translational clinical research on cellular tissue products for regenerative medicine and transplantation
Cryobiology is defined as the study of the subjects under effects of temperatures lower than normal physiologic ranges upon biological systems [19]
Summary
Cryopreservation of reproductive cells and tissues has become an increasingly important methodology for future fertility advantages [1,2]. Cryopreservation of testicular cells and tissue prior to any fertility compromising condition or therapy may allow for future tissue transplantation back to the autologous donor so that they may regain the ability to naturally conceive their own biological children [3]. Scientist regards to cryopreservation be crucial for enabling technology to the progression from preclinical and translational clinical research on cellular tissue products for regenerative medicine and transplantation. The need and advantages of tissue cryopreservation are widely recognized and well documented. These cells may be used to create new sperm outside the body through germ cell maturation protocols [4]. Investigational techniques for cryopreserving testicular tissue and cells have been tested and reported by several groups [5,6]; to our knowledge, a clinical-grade protocol for the cryopreservation of rat testicular cells or tissue has not been previously described
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