Abstract
The feasibility of utilizing crude glycerol as carbon source for heterotrophic growth of the green microalgae Chlorella pyrenoidosa and Coccomyxa subellipsoidea C-169 was investigated. The highest biomass concentration of C. pyrenoidosa (6.25 g L−1) and C. subellipsoidea C-169 (7.62 g L−1) was achieved in basal medium containing 5 and 10 g L−1crude glycerol. Compared to pure glycerol and glucose, the algal cells grown in crude glycerol media obtained a higher intracellular protein content, while the microalgal lipid consists of a large amount of unsaturated fatty acids. The most abundant fatty acids in C. pyrenoidosa and C. subellipsoidea C-169 were linolenic acid (C18:3) and palmitic acid (16:0). The cetane number (49.0) of both investigated algal species corresponds to the requirements of the Standard B100. Our results indicate that crude glycerol could provide a promising alternative feedstock for heterotrophic growth of these two microalgae.
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