Effect of countermeasure bodygear on cardiac-vascular-respiratory coupling during 6-degree head-down tilt: An earth-based microgravity study

Abstract

Prolonged exposure to microgravity causes physiological deconditioning in humans. Herein, a novel designed countermeasure gravitational load modulation bodygear has been developed to deal with the ill effects of the microgravity environment. The bodygear is designed to provide the wearer an axial loading from the shoulder to the feet that simulate Earth's gravity. The present study aims to evaluate the effect of bodygear on cardiac, vascular and respiratory systems during head-down tilt (HDT) microgravity analogue. In this, 30 healthy male subjects have volunteered and their average age, height and weight were 24.56 ± 3.87 yr, 168.4 ± 9.17 cm and 65.9 ± 10.51 kg respectively. The physiological signals such as electrocardiogram (ECG), blood pressure (BP) and respiration were recorded non-invasively using Biopac MP100. The signals were sampled at 1,000 Hz and processed using MATLAB 2018b. The signals were analysed in linear well as non-linear domains. The ECG and BP were used to derive R-R interval (RRI) and systolic blood pressure (SBP). The respiration time series (RSP) was derived by extracting R-peaks from the ECG signal and using these peaks to find the respiration amplitude. The non-linear domain analysis was used for the detection and quantification of information flow among the recorded signals. Repeated measure analysis of variance with Bonferroni post-hoc paired t-test was used for statistical analysis with the p < 0.05. The experimental results show that the 6-degree HDT activates the parasympathetic system and decreased the RRI effect on SBP (p = 0.005). Interestingly with the bodygear usage, the sympathetic system activated, mean RRI decreased (p = 0.018) and blood pressure increased (p = 0.031) as compared to baseline. Further, it was also observed that the effect of RRI on SBP (p = 0.029) and SBP on RRI (p = 0.012) was increased with bodygear as compared to HDT without bodygear. The conditional entropy technique aided in analyzing the effect of bodygear on information flow variation in the cardiovascular system of the human body.