Effect of Coptischinensis, Glycyrrhiza uralensis, and Fermented Glycine max Extract as Proactive Therapy for Atopic Dermatitis

Beom-Chan Park,Ki-Bong Kim,In-Jun Yang,Sang-Hyun Ahn

doi:10.3390/app11188380

Abstract

The aim of this study was to investigate the effect of the Coptis chinensis, Glycyrrhiza uralensis, and fermented Glycine max (3Hb) extract on lipid barrier recovery and the alleviation of atopic dermatitis (AD). The 3Hb extract was administered to lipid barrier-eliminated mice (3HbT) for 5 days. Subsequently, the effect of the 3Hb extract on general skin features and the regulation of filaggrin, inflammatory response, Th2 differentiation, and the skin micro-environment for defense, was evaluated. In the 3HbT, filaggrin was effectively recovered. The clinical skin score was significantly lower in the 3HbT compared with control groups. In addition, significant decreases in pH and TEWL as well as in the levels of kallikrein 7, PAR-2, TSLP, IL-4, Fc ε receptor, and phosphate-NF-κB p65 were observed in the 3HbT, compared with the other control groups. Further, compared with control groups, the 3HbT showed a significant increase in those of claudin, cathelicidin, TLR, and NHE-1. Our results indicated that the 3Hb extract effectively recovered filaggrin. Through the recovery of filaggrin, inflammation and the Th2 differentiation process can be regulated, and microenvironments for defense can be recovered. Therefore, we confirmed the potential of the 3Hb extract for use in the proactive therapy of AD.

Highlights

The skin acts as the primary barrier that separates the human body from the surrounding environment [1]
Upon comparison with the results of the standard compounds, 10 main molecules were identified in the high-performance liquid chromatography (HPLC) analysis: liquiritin (0.25 mg/kg), liquiritigenin
We found that the 3Hb extract contains known bioactive markers of 3Hb

Summary

Introduction

The skin acts as the primary barrier that separates the human body from the surrounding environment [1]. The skin is divided into the epidermis and the dermis. The epidermis protects the body from external threats, such as pathogens, toxins, and desiccation [2]. The epidermis is divided into the stratum corneum (SC), granular layer, spinous layer, and basal layer. The SC comprises a continuous layer of epidermal lipids that act create a layered structure, separating keratinocytes from other cells. Keratinocytes produce two membrane-circumscribed granules (keratohyalin granules and lamellar bodies) in the stratum granulosum and secrete them in the intercellular space. Keratohyalin granules are intracellular components of the SC. Filaggrin (FLG) is one such representative keratohyalin and is an intercellular component of the SC [3]. Epidermal lipids and keratohyalin granules determine the epidermal permeability

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