Abstract
This study was carried out to investigate the effec ts of copper sulphate and cobalt chloride on propagation of date palm cv. Ashgar in vitro . The rate of callus proliferation was significantl y higher in the medium supplemented with 2 µM copper sulphate and 2 µM cobalt chloride together (p<0.05). Addition of copper sulphate and Cobalt chloride to the medium was most effective for shoots regeneration from callus and enhanced regeneration frequency as well as number of shoots obtained per explant, the best result (7.12 shoot/explant) was o btained by using copper sulphate and Cobalt chlorid e at 2.0 µM. Also the results of this experiment indicat es that the maximum induction of roots can be achieved in the media containing both additives at 0.5 µM. The callus exhibited a decline in carbohydr ate contents, proteins, whereas total phenol content in creased at high concentration of Cu and Co. The greatest formation of chlorophylls a, b and total c hlorophyll was obtained in plantlets grown in the m edia containing both additives at 0.5 µM. As well as Max imum survivability was noticed for plants cultured in media that containing both copper sulphate and Coba lt chloride at 0.5 µM.
Highlights
Date palm (Phoenix dactylifera L.) of the family Arecaceae is a key plantation crop of many countries of arid regions of West Asia and North Africa (Al-Khalifah et al, 2012)
Comparing copper sulphate and Cobalt chloride, copper sulphate was found better for callus proliferation .The rate of callus proliferation was increased in the media contain Cobalt chloride at 2 μM statistical analysis did not show significant differences between different concentrations of cobalt sulfate and Control treatment on callus proliferation at the 0.05 level (Fig. 6A, C and Table 2)
The results clearly showed that the rate of High quality granular callus proliferation in this experiment was significantly higher in the medium containing both additives at the concentrations of 2 mg L−1 CuSO4 and 2 mg L−1 CoCl2 (Fig. 6D)
Summary
Date palm (Phoenix dactylifera L.) of the family Arecaceae is a key plantation crop of many countries of arid regions of West Asia and North Africa (Al-Khalifah et al, 2012). Second sources of the propagation are seeds, but it has many limitations like low rate of germination and progeny variations (Chand and Singh, 2004). The metals like cobalt, iron, manganese, copper and zinc are essential for plant life but are required in a very small or trace amounts and become toxic at higher concentrations (Hussein et al, 2010). Copper is a microelement that is essential for normal growth and development of plants. In plant organisms it performs very important physiological and biochemical functions. It takes part in the processes of photosynthesis, respiration, conversion of nitrogen compounds, transport of Science Publications
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: American Journal of Agricultural and Biological Sciences
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
