Effect of copper sulfate on liver damage induced by nano- zinc oxide in Cyprinus carpio

S.K.I Al-Taee,A.H.A Al-Hamdani

doi:10.33899/ijvs.2014.116942

Abstract

The effects of Copper Sulfate (CuSO4) on repaired the histopthological changes on liver of Cyprinus carpio exposed to nano zinc oxide (N-ZnO) was determined in this study.Fish weight 150±10 g divided in to four groups.Fish in first group kept in dechlorinated water while the fish in second group exposed to copper sulfate CuSO4 for 1 hour and in third group the fish was exposed to sub lethal concentration of N-ZnO (30ppm) for 7 days while the fish in fourth group exposed to sub lethal concentration of N-ZnO (30ppm) with CuSO4 (0.3mgL) for 1hour and then exposed continuously to N-ZnO for 7days. The microscopic examination for the liver done through 24 hour and 7 days from the experiment, which revealed vacuolar degeneration in hepatic cells in fish exposed to N-ZnO for 24 hour also there were dilatation of sinusoid and infiltration of inflammatory cells in liver tissue of fish exposed to to N-ZnO with CuSO4 for 1 hour, there were slight hemorrhage in hepatic tissue and enlargement of hepatic cell in both groups exposed to N-ZnO and CuSO4 for 1 and 24 hour, while the microscopically examination of liver in fish exposed to N-ZnO for 7 days revealed sever pathological lesions characterized by hyperatrophy and necrosis in pancreatic tissue. This study concluded that N-ZnO can cause oxidative stress and usage of CuSO4 at 0.3 mg/L can decrease the toxic effects of N-ZnO liver tissue

Highlights

Copper is trace element essential for many physiology development and growth [1], it play an important roles in hemoglobin synthesis,connective tissue synthesis and enzymes involved in cellular respiration as well as in to some structural of protein [2]
No histopathological changes were observed in the livers of control fish, while the microscopic examination of the liver of fish in groups 2nd and 3rd at 1 hour revealed slight hemorrhage in hepatic tissue and swelling of hepatic cells fig. [1], there was hepatic cell vaculation in fish exposed to N-ZnO at 24 hour fig
These lesions become more sever on fish which are exposed continuously to NZnO for 7 day fig. [5] which revealed hyper atrophy, edema and enlargement of sinusoid with infiltration of inflammatory cells and piknotic hepatic cells with necrosis in pancreatic tissue, Fig. 1: liver of fish exposed to CuSO4 0.3mg/Lfor 1hour,revealed slight hemorrhage(a) H&E 420X

Summary

Introduction

Copper is trace element essential for many physiology development and growth [1], it play an important roles in hemoglobin synthesis,connective tissue synthesis and enzymes involved in cellular respiration as well as in to some structural of protein [2]. Fish species show beheavioral and locomotor changes and different mortality rate in responses to varing concentration of copper sulfate [8,9] observed that CuSO4 cause damage to the gill epethlium, liver tissue, kidney and spleen of fish, osmoregulation disturbances, immunosuppression and alter blood parameter [9-11]. Liver and gill might be the target tissue for N-ZnO toxicity [16,17] obsereved that N-ZnO at concentration 30 ppm cause necrosis in both liver and pancreatic tissue in Cyprinus carpio, [18] reported that N-ZnO cause oxidative stress (OS) in gill, liver, brain, intestine of Cyprinus carpio and cause changes in antioxidant defense mechanism, there were histopathological changes in gill of Cyprinus carpio treated with different concentration of N-ZnO for 21 days include hyperplasia and degeneration of epithelial cell, vacuolization [19], N-ZnO toxic for embryos and may cause pericardial edema in zebrafish (Danio rerio) embryos and decrease hatchery rate [20]

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