Effect of Continuous Feeding of Ayu-Narezushi on Lipid Metabolism in a Mouse Model of Metabolic Syndrome.

Takeshi Nishida,Akira Noguchi,Hideki Hatta,Koji Nomura,Johji Imura,Koichi Tsuneyama,Takahiko Nakajima,Yasuhiko Tago,Makoto Fujimoto,Takashi Minamisaka,Juei Tang Cheng

doi:10.1155/2021/1583154

Abstract

Ayu-narezushi, a traditional Japanese fermented food, comprises abundant levels of lactic acid bacteria (LAB) and free amino acids. This study aimed to examine the potential beneficial effects of ayu-narezushi and investigated whether ayu-narezushi led to improvements in the Tsumura Suzuki obese diabetes (TSOD) mice model of spontaneous metabolic syndrome because useful LAB are known as probiotics that regulate intestinal function. In the present study, the increased body weight of the TSOD mice was attenuated in those fed the ayu-narezushi-comprised chow (ayu-narezushi group) compared with those fed the normal rodent chow (control group). Serum triglyceride and cholesterol levels were significantly lower in the Ayu-narezushi group than in the control group at 24 weeks of age. Furthermore, hepatic mRNA levels of carnitine-palmitoyl transferase 1 and acyl-CoA oxidase, which related to fatty acid oxidation, were significantly increased in the ayu-narezushi group than in the control group at 24 weeks of age. In conclusion, these results suggested that continuous feeding with ayu-narezushi improved obesity and dyslipidemia in the TSOD mice and that the activation of fatty acid oxidation in the liver might contribute to these improvements.

Highlights

Metabolic syndrome (MS) is a serious global health problem that is expected to worsen in the future because of the increasing prevalence of obesity, sedentary lifestyles, and unbalanced diets [1,2,3]
There was a tendency of attenuation in the increase of mean body weight starting approximately at 11 weeks of age in the ayu-narezushi group compared with the control group (Figure 1)
Comparison of Histopathological Findings in Visceral Fat in the Two Groups. e size of visceral adipocytes was remarkably smaller in the ayu-narezushi group than in the control group at 24 weeks of age (Figures 2(a) and 2(b)); no remarkable difference was observed in their size between the two groups at 8 weeks of age (Figures 2(c) and 2(d)). e quantification of the images captured at identical magnification revealed that the mean number of visceral adipocytes at 24 weeks of age was significantly higher in the ayu-narezushi group (100.3 ± 18.2 cells) than in the control group (85.2 ± 14.5 cells) (Table 2, P < 0.05)

Summary

Introduction

Metabolic syndrome (MS) is a serious global health problem that is expected to worsen in the future because of the increasing prevalence of obesity, sedentary lifestyles, and unbalanced diets [1,2,3]. MS initiates with visceral fat accumulation and progresses to insulin resistance, abnormal lipid metabolism, hypertension, and various other diseases throughout the body [4]. MS may lead to/ related to type 2 diabetes mellitus, vascular inflammation, atherosclerosis, and renal, liver, and heart diseases. Numerous studies have reported the beneficial effects of probiotics in improving dysbiosis, and probiotics have been increasingly introduced as health-promoting supplements in our daily diet. Beneficial commensal microbes, such as Bifidobacterium spp. and Lactobacillus spp. have long been consumed as supplements [8, 9]

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Discussion

Conclusion