Abstract
Acanthamoeba keratitis among contact lens wearers is being reported with increasing frequency. Since chemical disinfection is an important part of the contact lens care regimen, we investigated the activity of hydrogen peroxide (0.5%-3.0%), a formulation of chlorhexidine gluconate (0.004%), and thimerosal (0.0025%) against the cyst forms of pathogenic Acanthamoeba species. Test organisms included Acanthamoeba castellanii strain CCAP 1501/2g (30868, American Type Culture Collection, Rockville, MD) and Acanthamoeba polyphaga strain SHI that were maintained at 30?C in a semidefined liquid medium [1]. Mature cysts were produced from these cultures with use of a constant pH encystment medium [1]. When chlorhexidine gluconate was being tested, all glass-
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