Abstract
The present study was designed to define whether dietary conjugated linoleic acid (CLA) could affect antioxidant enzymes including superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), catalase (CAT), and glutathione S transferase (GST), and the level of malondialdehyde (MDA), a marker of lipid peroxidation, in the small intestine and liver from broiler chickens. A total of twenty-four 3 wk-old male broiler chickens were assigned to three dietary treatments (1.5% corn oil, 0.75% corn oil plus 0.75% CLA, and 1.5% CLA, isocalorically), and fed a grower-finisher diet from 22 to 35 days. In the small intestinal mucosae, the specific activities of SOD, GSH-Px, CAT, and GST, and the level of MDA were not substantially influenced by dietary CLA. In the liver, the specific activities of SOD, GSH-Px, and GST, and the level of MDA were also unaffected by dietary CLA at the level of either 0.75% or 1.5% compared with corn oil at the level of 1.5%. However, the broiler chickens fed the diet containing 1.5% CLA resulted in a significant increase in peroxisomal CAT activity and a marked decrease in total lipid and non-esterified fatty acids (NEFA) from liver tissues compared with those fed the diet containing 1.5% corn oil. In conclusion, ability of CLA to increase hepatic CAT activity suggest that dietary CLA may affect, at least in part, antioxidant defense system as well as lipid metabolism in the liver of broiler chickens.
Highlights
Conjugated linoleic acid, a group of positional and geometric isomers derived from linoleic acid, has attracted considerable attention for the last decade due to its myriad of biological actions against cancer, obesity, arteriosclerosis, and oxidative stress in several animal models (Ha et al., 1990; Whigham et al, 2000)
The broiler chickens fed the diet containing 1.5% corn oil (CORN) showed 2.3% higher weight gains than those fed the diet containing 1.5% conjugated linoleic acid (CLA)
Effect of CLA on the specific activity of antioxidant enzymes and lipid peroxidation To determine whether dietary CLA affects antioxidant ability in the small intestine and liver from the broiler chickens, we determined the specific activities of superoxide dismutase (SOD), GSH-Px, SOD, CAT, and glutathione S transferase (GST), and the level of MDA, a marker of lipid peroxidation
Summary
Conjugated linoleic acid, a group of positional and geometric isomers derived from linoleic acid, has attracted considerable attention for the last decade due to its myriad of biological actions against cancer, obesity, arteriosclerosis, and oxidative stress in several animal models (Ha et al., 1990; Whigham et al, 2000) Their beneficial effects have raised a great attention to obtain CLA-containing food, since the only small amounts of CLA is naturally existed in meat and dairy products of ruminants (Wang et al, 2002). Recent several studies with experimental animals have suggested that CLA had a more direct effect on the increased lipid peoxidation, which may contribute to the anti-tumorigenic and anti-obesity (Basu et al, 2000) These evidences provided that dietary CLA, at least somewhat, played a role in changes in antioxidant or lipid peroxidation, depending on the availability of CLA in tissues and circulation in animals. To reveal the effect of dietary CLA on the antioxidant ability, the present study was conducted to examine the specific activity of antioxidant enzymes such as GSH-Px, SOD, CAT, and GST, and the level of MDA in the small intestine and liver from broiler chickens
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