Abstract
The effects of feeding diets with conjugated linoleic acid (CLA) to Astyanax altiparanae for different periods were investigated on carcasses’ fatty acid profile and nutritional quality. The trial was laid out in a complete randomized design with eight feeding periods (0, 7, 14, 21, 28, 35, 42, and 49 days) and four replicates. A total of 240 fish (3.4 ± 0.3 g) were distributed into four aquariums and fed with the diet containing 32% crude protein, 15 MJ kg-1 gross energy, and 2.5% of CLA. Feeding CLA diet for 49 days resulted in the higher deposition of CLA, CLA isomers, and docosahexaenoic acid (DHA). The isomer c9,t11 was highly deposited than the t10,c12. Polyunsaturated fatty acids (PUFA), n-3, and n-6 increased with feeding period, whereas saturated (SFA), monounsaturated, and medium-chain fatty acids decreased. PUFA/SFA ratio, DHA/EPA, EPA+DHA, and thrombogenicity index increased linearly. Whereas atherogenicity index reduced, hypocholesterolemic/hypercholesterolemic ratio increased in quadratic effects over feeding time. No difference was observed for the n-6/n-3 ratio. Feeding A. altiparanae with 2.5% of CLA for a minimum of 35 days improves the fatty acid profile and provides a commercial product with good nutritional quality and functional benefits.
Highlights
Conjugated linoleic acid (CLA) refers to a group of geometric and positional isomers of linoleic acid (18:2n-6) with two conjugated double bonds
Feeding duration significantly modified the incorporation in % of total fatty acids of CLA and its isomers and the fatty acid profile of the fish carcasses (Table 2)
The CLA isomers showed distinct incorporation patterns, which included a linear effect for t10,c12 and a quadratic effect for c9,t11 (Figure 1)
Summary
Conjugated linoleic acid (CLA) refers to a group of geometric and positional isomers of linoleic acid (18:2n-6) with two conjugated double bonds. The physiological effects and health benefits of CLA for humans, which were thoroughly reviewed by Chen and Park (2019), are primarily linked to the 18:2 cis-9, trans-11 (c9,t11), and 18:2 trans-10, cis-12 (t10,c12) isomers. Daily CLA intake can vary between countries and Oliveira et al, Bol. Inst. EFFECT OF CONJUGATED LINOLEIC ACID FEEDING PERIODS
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