Effect of concentrated growth factor on osteoblast cell response

Abstract

ObjectiveThe purpose of this study was to assess the effect of concentrated growth factor (CGF) on osteoblast cell proliferation and differentiation. MethodsSaOS-2 osteoblast-like cells were cultured on titanium discs. The disc surfaces in the test group were treated with CGF serum, and those in the control group were left untreated. Cell counts, cell proliferation, and osteocalcin (OCN) levels were evaluated on days 1 and 3, and the alkaline phosphatase (ALP) activity was assessed on days 3, 7, and 14. ResultsThe proliferation values were significantly higher in the test group than in the control on days 1 and 3 (P<0.05). ALP activities increased gradually in both groups from day 3 to day 14, but the ALP values were significantly higher in the test group than in the control in all periods (P<0.05). The OCN level on day 1 was significantly higher (P<0.05) in the test group, and no statistically significant difference was found between the two groups on day 3 (P<0.05). A significant decrease was observed in OCN activity on day3 in comparison with day 1 in the control group (P<0.05). DiscussionThe results suggest that CGF can efficiently stimulate the proliferation and differentiation of osteoblast cells, thereby improving the healing process around implants.