Effect of concentrate enriched with palmitic acid versus rapeseed oil on dairy performance, milk fatty acid composition, and mammary lipogenic gene expression in mid-lactation Holstein cows

Abstract

This study was performed to characterize the effect of a concentrate supplemented with free palmitic acid (4% on a DM basis; PA) or rapeseed oil (4% on a DM basis; RO) compared with a no-added-lipid control concentrate (CT) on the performance of dairy cows fed a corn silage-based diet over a 9-wk period. After a 3-wk pre-experimental period, 54 Holstein cows were randomly allocated to 3 experimental treatments to receive forage ad libitum with a fixed amount of CT, RO, or PA (8 kg/d for 2-yr-old primiparous; 10 kg/d for older cows). During the experiment, dry matter intake, milk yield and composition, fatty acid (FA) yields and FA profile, and feed efficiency were determined. At wk 9 of the experimental period, the mRNA levels of 10 genes involved in lipid metabolism in mammary tissue biopsy samples were measured. Compared with CT, RO and PA increased forage intake. Compared with CT, RO increased concentrate intake, the value being intermediate for PA. Compared with CT, RO increased milk yield (+2.0 kg/d) and decreased milk fat and protein content (-3.8 and -1.2 g/kg, respectively), whereas PA increased milk fat content (+4.1 g/kg). Compared with CT and RO treatments, PA increased milk fat yield (+179 g/d) and 3.5% fat-corrected milk and energy-corrected milk output (+2.8 and +2.3 kg/d, respectively), and thus improved feed efficiency (+7.3%). Compared with CT treatment, RO increased milk contents of the sum of >C16 FA, monounsaturated FA, polyunsaturated FA, trans FA, and n-3 FA, whereas PA decreased these FA contents (except n-3 FA) and also decreased n-6 FA. The variations in milk fat yield and content and FA secretion at wk 9 were not associated with modifications in mammary expression of 10 genes involved in major lipid pathways, except for the transcription factor PPARG1, which tended to be higher in PA versus RO treatment. This study demonstrated that PA improved milk fat yield and feed efficiency compared with RO and suggests that factors other than gene expression, such as substrate availability for mammary metabolism or other levels of regulation (transcriptional, posttranscriptional, translational or posttranslational), could play a key role in milk fat and FA responses to changes in diet composition in cows.