Effect of compound Guizhu capsule on phosphate and tension homology deleted on chromsome ten and murine double mimute 2 gene expression in lung cancer of mice

Abstract

ObjectiveTo observe the inhibitory effect of the Chinese herbal compound Guizhu capsule (CGZC) on lung cancer and explore the possible mechanism underlying its actions by evaluating its effects on the expression of phosphate and tension homology deleted on chromsome ten (PTEN) and murine double mimute 2 (MDM2) in lung cancer model mice. MethodsA mouse model of transplanted lung cancer was established by subcutaneous inoculation of cancer cells into the axillae of mice. Twenty-four hours later, the mice were weighed and randomly divided into the model control group, cisplatin group (DDP group), and high, moderate and low dosage CGZC groups, with ten mice in each group. The control mice received an equal volume of distilled water. DDP was intraperitoneally injected at 1 mg/kg in the DDP group, once a day for 3 days. CGZC diluted with distilled water was administered at 20 g/kg (10 times the clinical adult dosage) in the high-dose group, 10 g/kg (5 times the clinical adult dosage) in the moderate-dose group and 5 g/kg (2.5 times the clinical adult dosage) in the low-dose group once a day for 10 days. On the 11th day, the mice were weighed and killed. The tumor tissues were weighed and the tumor inhibition rate was calculated. PTEN and MDM2 protein expression were detected by immunohistochemical analysis of tumor tissues. ResultsThe CGZC high- and moderate-dose groups showed a significant inhibitory effect on tumor growth (P < 0.05); there was no significant difference between the high dose group and the DDP group (P > 0.05). The CGZC high-dose group also showed enhanced expression of PTEN protein (P < 0.01) and decreased expression of MDM2 protein (P < 0.01) in lung cancer cells of the mice. There was no significant difference between the high dose group and the DDP group. ConclusionCGZC has a significant inhibitory effect on transplanted lung cancer in mice. The mechanism may involve reducing expression of PTEN and decreasing the expression of MDM2 in the lung cancer tissues of the mice.