Abstract
Single nucleotide polymorphisms (SNPs) in genes that control folate uptake and metabolism may have an important effect on chromosomal stability. The present study investigated the effect of common SNPs in some of these critical genes on frequency of lymphocytes with micronuclei, a biomarker of chromosome breakage or loss. 164 individuals (94 males and 70 females) of different age ranging from 18 to 73 years participated in this study. Polymorphisms in GCPII (C1561T), RFC (G80A), MTR (A2756G), MTRR (A66G and C524T), TS (tandem repeats, 6 bp deletion in 3′-UTR region) and MTHFR (C677T and A1298C) were detected using PCR-based methods. Frequency of binucleated (BN) lymphocytes containing one or more micronuclei (BN-MN) was determined using the cytokinesis-block micronucleus (CBMN) assay and adjusted for the effects of age and gender. We did not find any significant association between BN-MN frequency and the common SNPs in GCPII, MTRR, TS and MTHFR genes. BN-MN frequency in individuals who carried at least one copy of the rarer G allele for MTR (A2756G) or were homozygotes for the more common G allele for RFC (G80A) had a 14% or 19% lower BN-MN frequency compared to the alternative genotypes for that SNP respectively. It was evident from genotype combination analyses that BN-MN frequency per 1000 BN cells was highest in those with the combined MTR (2756) AA and RFC (80) GA or AA genotype (13.6‰) and lowest in those with the combined MTR (2756) AG or GG and RFC (80) GG genotypes (9.5‰) ( P trend = 0.015). The RFC G80A and MTR A2756G polymorphisms and their combinations may be important variables that substantially affect lymphocyte BN-MN frequency in this South Australian cohort.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
More From: Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.