Abstract

In this study two sources of resistance to Barley yellow dwarf virus (BYDV), both originating from Thinopyrum intermedium, were combined in a single genotype, line ZT, using GISH (genomic in situ hybridization) and a new molecular marker of the partial resistance gene Bdv2. Susceptible wheat cv. Sunstar, the translocation line TC14 carrying Bdv2, the addition line ZH with the group‐2 chromosome arm carrying resistance, and line ZT with both resistances were inoculated with five strains of Barley yellow dwarf virus‐PAV. The tests confirmed the resistance of TC14 and ZH and revealed an additive effect of the two sources of resistance in ZT. The resistance of line ZT was characterized by a proportion of infected plants significantly lower than the parental lines TC14 and ZH (42% vs. a mean of 76% for the parents) and a very low virus titre (area under the virus concentration progress curve of 1·2 vs. a mean on 6·3 for the parents).

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