Abstract
The emergence of multidrug-resistant tuberculosis (MDR-TB) is a matter of global concern. The use of mycobacteriophages alone or in combination with antibiotics could be used as an alternative approach to treat drug-resistant Mycobacteria. The aim of the study was the evaluation of the effect of an isolated mycobacteriophage in combination with Isoniazid, Rifampicin, Streptomycin, and Ampicillin against Mycobacterium tuberculosis and Mycobacterium smegmatis. Determination of the MIC of the above drugs for each strain was done by the standard tube dilution method. Further, the inhibitory effect of varying drug concentrations in combination with different dilutions of the mycobacteriophage was studied. The growth inhibition pattern for both the organisms was also studied in the presence of different dilutions of mycobacteriophage by turbidometric and resazurin dye reduction method. The killing pattern of drugs, at concentrations below the MIC, when combined with mycobacteriophage, was determined. The phage induced lysis of bacteria assisted in decreasing the inhibitory concentration of the drugs.
Highlights
The emergence of multidrug-resistant tuberculosis (MDR-TB) is a matter of global concern
The use of internationally recommended treatment regimens, mycobacteriophages alone or in combination with antibiotics according to WHO guidelines, must be scaled up and could be used as an alternative approach to treat drugresistant Mycobacteria
The aim of the study was the evaluation of the effect of an isolated mycobacteriophage in combination with Isoniazid, Rifampicin, Streptomycin, and Ampicillin against Mycobacterium tuberculosis and strengthened to prevent further second-line drug resistance and spread of XDR-TB
Summary
The emergence of multidrug-resistant tuberculosis (MDR-TB) is a matter of global concern. The aim of the study was the evaluation of the effect of an isolated mycobacteriophage in combination with Isoniazid, Rifampicin, Streptomycin, and Ampicillin against Mycobacterium tuberculosis and Mycobacterium smegmatis. The aim of the study was the evaluation of the effect of an isolated mycobacteriophage in combination with Isoniazid, Rifampicin, Streptomycin, and Ampicillin against Mycobacterium tuberculosis and strengthened to prevent further second-line drug resistance and spread of XDR-TB. The inhibitory effect of varying drug second-line drugs, suggests that urgent measures are concentrations in combination with different dilutions of the mycobacteriophage was studied. Needed to improve the rational use of quality-assured second-line drugs, new anti-TB drug regimens and better diagnostic tests for TB and MDR-TB. As these drugs are costly and more toxic, there is a requirement of urgent action for the improvement of the quality of diagnosis and the treatment of drug-resistant TB (Website 1)
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