Abstract
To assess the effect of cold stress on the levels of minerals (C, N, P, K, Ca, Mg, Mn, Zn, Cu and Fe) and water soluble vitamins (B1, B2, B3, B5, B6, B9 and C) in spinach, an experiment was set up under greenhouse conditions and compared to field results. Levels of macro and micronutrients, except iron in spinach were unaffected by growth conditions and cold stress. The abundance of water soluble vitamins were most pronounced in greenhouse grown plants exposed to 14 and 21 days of cold stress. Significant increases in vitamin C (from 22.73 to 34.47 mg 100 g-1 dry matter) accumulation in stressed plants were observed, thus highlighting its role in plant defense mechanisms. Results indicate that cultivation of leafy vegetables in a greenhouse with low temperature (4 to 7°C) exposure for a shorter period (14 to 21 days) before harvest can improve the nutritional quality. Water soluble vitamin (WSV) contents of field- and greenhouse-grown spinaches.
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