Abstract
SummaryThe effects of short-to medium-term cold storage (3-4°C) on the maintenance of embryogenic capacity and the germination and conversion of somatic embryo cultures of Camellia were investigated. Four culture lines maintained by repetitive embryogenesis were used: three C. japonica lines and one C. reticulata cv. Mouchang line. Increasing cold storage time (3, 6, 9, and 12 months), decreased the frequency of surviving embryo clusters and the mean number of surviving embryos per cluster. Eight week old embryogenic cultures were stored for one or two months, after which secondary embryos were isolated from the parent embryos and transferred to the germination medium. Two months of chilling significantly improved plantlet conversion abilities of secondary embryos, with rates ranging from 76–100% (depending on the embryogenic line) against 32–65%, respectively, in the absence of cold treatment. The incidence of secondary embryogenesis during germination also decreased after cold treatment. Cold storage of...
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
