Effect of cold exposure on plasma glucose and acetate turnover rates in sheep

Abstract

Isotopic dilution methodology of [U-13C]glucose and [1-13C]Na-acetate were performed simultaneously as primed continuous infusions to determine plasma glucose and acetate metabolism in sheep during thermoneutral (TN) and cold exposure (CE, 2−4°C). The experiment was performed using crossbred (Corriedale × Suffolk) sheep (n = 5; 3 male and 2 female; ~2 years old, 38 ± 2.5 kg of initial bodyweight). The animals were offered mixed hay of orchardgrass and reed canarygrass (40 : 60) 62 g/kg0.75/day with ad libitum water access. Concentration of plasma glucose was determined enzymatically using the glucose oxidase method. Plasma concentration of non-esterified fatty acids (NEFA) and glucose were higher (P = 0.01 and P = 0.05 respectively) during CE than TN. Plasma concentration and the turnover rate of acetate were numerically higher (P = 0.09 and P = 0.25 respectively) during CE than during TN. Plasma glucose turnover rate was higher (P = 0.02) during CE than TN. The present findings suggested that plasma acetate turnover rate tended to be elevated during CE, with further more extensive studies required to clarify the significance.