Effect of Cold Exposure and Photoperiod on Diapause Termination of the Predatory MiteEuseius finlandicus(Acari: Phytoseiidae)

Abstract

The effect of cold storage and photoperiod on diapause termination of the predatory mite Euseius finlandicus was studied under laboratory conditions. Females in diapause that developed under a short-day photoperiod of L:D 8:16 h and 20°C were exposed for 1–13 wk to 5°C and subsequently maintained under L:D 8:16 h and 20°C, after which the percentage of females terminating diapause was recorded. After 1–4 wk at 5°C, the number of days required for diapause termination in 50% of females (TA50) was similar to that of the control mites maintained continuously at L:D 8:16 h and 20°C. This indicates that cold is not a prerequisite for the completion of diapause development. Furthermore, when mites were maintained for longer periods (7, 10, or 13 wk) at 5°C, TA50 was significantly longer than in the control mites. This may indicate an adverse effect of cold storage on the completion of diapause development or on postdiapause development. However, the longer exposures to cold resulted in synchronous diapause termination. When diapause females were maintained under a series of different photoperiods and 20°C without previous cold exposure, diapause was terminated rapidly under the photoperiods of L:D 16:8, 14:10, and 13:11 h. In contrast, under the shorter-day photoperiods of LD 8:16, 10:14, and 12:12 h, diapause was maintained and eventually ended spontaneously after a long period of time. These results may be useful for the long-term storage of this predatory mite, which could be required for use in biological control programs.