Effect of Colchicine treatment on the cariology and morphology signs of Polemonium caeruleum L.

Alina Glazunova,Firdaus Hazieva,Tatiana Samatadze,D Fayzrakhmanov,B Ziganshin,R Shaydullin,F Nezhmetdinova

doi:10.1051/bioconf/20201700210

Abstract

The effect of colchicine treatment of P. caeruleum on morphological and economically valuable traits, pollen size and fertility, and karyotype of also was studied. Colchicine treatment increased in the size of pollen by 13 % compared to the control, pollen fertility decreased by 19 %. Stained chromosomes of the karyotype were identified, their sizes were determined for the first time, and karyotype formulas for diploid K = (5m + 2sm + 2st) and tetraploid forms K = 2 (10m + 4sm + 4st) were compiled. As a result of colchicine treatment, the plant height decreased by 35 %, the stem thickness by 47 %, the number of generative shoots 18 %, the leaf length 23 % and width increased 22 %, compared to the control. The productivity of raw by 32 % and seeds of tetraploid plants exceeds diploid plants by 22 %.This research was supported government as signment (No. 0576-2019-0008).

Highlights

IntroductionPolemonium coeruleum L. – perennial herbaceous herbal plant of Polemoniaceae, height 35–120 сm
Polemonium coeruleum L. – perennial herbaceous herbal plant of Polemoniaceae, height 35–120 сm.The flowers are large, bright blue, the stem is hollow, solitary, straight (Fig. 1)
3.3 Morphometric and economically valuable characteristics. It was revealed tetraploid plants significantly differ from diploid plants in morphological characters

Summary

Introduction

Polemonium coeruleum L. – perennial herbaceous herbal plant of Polemoniaceae, height 35–120 сm. The flowers are large, bright blue, (rather white), the stem is hollow, solitary, straight (Fig. 1). It blooms from the second year of life, in the first half of summer for. This plant is important in traditional Chinese and Russian medicine. Chemical mutagenesis is a simple approach used to create a mutation in economically valuable plants, for improvement of their agronomic parameters. A chemical mutagenesis is a simple approach that is used to produce mutations in economically valuable plants, to improve their useful economic characteristics. Datura stramonium variety “Beshipny” was obtained as a result of treatment seedlings with a 0.2 % solution of colchicine [14]. Two new varieties of Calendula officinalis the “Zolotoe more” and the “Rajskij sad” was develop by colchicines treatment [15]

Materials and methods

Pollen characteristics

Karyotyping

Morphometric and economically valuable characteristics

Conclusion