Effect of Coffea arabica L on antibiotic-resistant Pseudomonas aeruginosa

Abstract

Pseudomonas aeruginosa is considered an opportunistic bacteria that causes many risks and because of its resistance to antibiotics, the virulence of this bacteria has increased. To get rid of the problem of bacterial resistance to antibiotics, effective medicinal plants were used to get rid of pathogenic bacteria so during this study, 200 samples were collected from patients with wounds and burn from Al-Sadiq Teaching Hospital in Babylon Governorate, distributed to 130 burn samples and 70 wound samples. The samples were cultured with specific and differential media. Where 30(15%) samples of P. aeruginosa were obtained and the diagnosis was confirmed by microscopy and biochemical tests and confirmed by VITEK-2 Compact. The sensitivity test showed its resistance to many antibiotics piperacillin 15/30 (50%) , piperacillin - tazobactam (4/30(13.3%), ceftazidime and cefepimewere each one (25/30(83.3%), Aztreonam 8/30(26.6%), Imipenem5/30(16.6%), Gentamicin 12/ 30(40%) 30, Tobramycin9/30( 30%), Amikacin 6/30(20%), ciprofloxacin 13/30(43.3%), ofloxacin 5/30(16.6%). Because of the role of alternative medicine in combating antibiotic-resistant bacteria, the effectiveness of the coffee plant was measured against antibiotic-resistant P. aeruginosa was sensitive to the coffee aqueous extract, Coffea arabica Linn. (Rubiaceae (.The properties of the active substances in coffee were determined using characteristic techniques UV-Visible Spectra analysis, X-ray diffraction (XRD) and Fourier Transform Infrared Spectroscopy (FTIR). The study aims to investigate the effectiveness of the Arabica coffee plant as a bacterial antibiotic to challenge the problem of pathogenic bacterial resistance to the used antibiotics.