Effect of Coconut Water on the Growth of Gluconacetobacter xylinus Y15

Abstract

The effect of coconut water contents, sucrose and ammonium sulfate concentrations, cultivation modes on the growth of Gluconacetobacter xylinus Y15 was investigated. When 25%(V/V) coconut water, 3g/L (NH4)2SO4 was added into the medium without sucrose, and G. xylinus Y15 was cultivated in shake flask culture at 130r/min for 24h, the cell number could reach as high as 4.9×108CFU/mL, while the cell number was only 4.9×104CFU/mL when cultivated in static culture. The growth curves of G. xylinus Y15 in coconut water medium and HS [ medium in shake flask culture were compared. The lag phase of G. xylinus Y15 in coconut water medium was 20h shorter than in HS medium, and the log phase was 20h longer.