Effect of cobra venom factor on the immune function of the pig to monkey liver xeno-transplantation

Abstract

Objective To explore the effect of high purity venom factor (CVF) and other factors on immune function after pig to monkey liver xeno-transplantation. Methods To establish the pig to monkey heterotopic auxiliary liver transplantation model, the occurrence of super acute rejection (HAR) and delayed rejection (DXR) was observed after liver transplantation while interfered by high purity CVF. The expression of IgG, IgM and C3 protein in liver tissues was detected by Western blotting at different time points. Results The donor liver used for the adsorption all occured HAR, respectively of 10, 12, 11, 9, 13 minutes, the average time was (11.00±1.41) minutes. The pathological findings were ischemic necrosis and thrombosis. After CVF and other multiple factors, all the recipient monkey liver did not observe HAR and DXR until death, the IgG, IgM and C3 protein deposition in liver tissue after the death of the receptor were lower than the donor liver used for the adsorption, which is statistically significant difference (P=0.026, 0.047, 0.032). Conclusion CVF and other multiple factors can effectively overcome the occurrence of HAR in pig to monkey liver xeno-transplantation, and has the potential to prevent or delay the DXR. Key words: Xenograft; Snake venom factor; Rejection