Abstract
In vivo experiments in mice demonstrated that 5% CO 2 content in the air inhaled did not change the labelling in autoradiograms from animals injected with [ 3H]uridine, [ 3H]orotic acid, [ 3H]hypoxanthine, [ 3H]lysine or [ 3H]cytidine. At 20% CO 2 content there was a significant decrease in labelling of brain cells with [ 3H]uridine and [ 3H]cytidine, but not following [ 3H]lysine; there was no labelling of nerve cells with [ 3H]orotic acid or [ 3H]hypoxanthine, but a control group was not included. The labelling of choroid plexus and hepatocytes was independent of the CO 2 concentration. A comparison of in vivo and in vitro experiments at 20% CO 2 content showed a similar significant decrease in labelling of brain cells with [ 3H]uridine and [ 3H]cytidine. It is concluded that a metabolic change is the most appropriate explanation of the CO 2 effect.
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