Abstract
Brain-derived neurothrophic factor (BDNF) is a neurotrophin expressed in different tissues and cells, including neurons, endothelial cells, leukocytes, megakaryocytes and platelets. Modifications of BDNF in plasma and/or in serum are associated with neurodegenerative and psychiatric disorders, cardiovascular diseases, metabolic syndrome and with mortality risk. Indeed, changes in blood levels of BDNF may reflect those of its tissue of origin and/or promote pathological dysfunctions. The measurement of BDNF amount in plasma or in serum has been characterized with particular attention in the impact of different anti-coagulants, clotting duration, temperature (≤21 °C) and delay in blood sample centrifugation as well as in stability of storage. However, the influences of normothermic conditions (37 °C) and of clotting duration on BDNF levels in human serum have not been investigated yet. Here, we showed that time and temperature during serum preparation could be taken into consideration to assess the association and/or impact of BDNF levels in the occurrence of pathological conditions.
Highlights
We read with great interest the article “Stability of Brain-derived neurothrophic factor (BDNF) in Human Samples Stored Up to 6 Months and Correlations of Serum and EDTA-Plasma Concentrations” published by Polyakova et al in International Journal of Molecular Sciences on 3 June 2017 in the Special Issue, “Brain-Derived Neurotrophic Factor”
We evaluated the influence of temperature (RT and 37 ◦C) on BDNF levels in human serum in relation to different clotting time-points and its release from platelets
The levels of BDNF in serum obtained after incubation of blood at 37 ◦C increased faster than in samples obtained at room temperature (RT)
Summary
We read with great interest the article “Stability of BDNF in Human Samples Stored Up to 6 Months and Correlations of Serum and EDTA-Plasma Concentrations” published by Polyakova et al in International Journal of Molecular Sciences on 3 June 2017 in the Special Issue, “Brain-Derived Neurotrophic Factor”. Many articles focused their attention on confounders of serum BDNF levels, analyzing the influence of clotting duration, storage time and temperature conditions of samples [6,7,8,9]. To maintain blood in normothermic conditions during serum preparation may be relevant in this experimental setting since BDNF is released by platelets during clotting.
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