Abstract
Earlier results from our laboratory have revealed that the inotropic response to ouabain was depressed in chronically diabetic rat heart (1). In this study we examined the effect of chronic streptozotocininduced diabetes (3 months) on [ 3H]ouabain binding in the rat heart. Scatchard analysis of [ 3H]ouabain binding to membrane preparations of rat left ventricle revealed two classes of binding sites; a high affinity/low capacity and a low affinity/high capacity binding site. The maximum number of binding sites of the high and low affinity binding sites in membrane preparations obtained from the chronically diabetic rats was significantly (p < 0.05) reduced to 60.4 and 48.8% of controls, respectively. The dissociation constant of the high and the low affinity binding sites in the chronically diabetic rat heart, compared to controls, was significantly (p < 0.05) increased and decreased, respectively. These results suggest that the decreased inotropic response of ouabain in the intact tissue obtained from chronically diabetic rats (1) may be related to a decreased number of ouabain binding sites.
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