EFFECT OF CHRONIC NEUROGENIC PAIN ON FUNCTIONAL ACTIVITY OF THYROID GLAND IN MALE MICE WITH TRANSPLANTABLE B16/F10 MELANOMA

Abstract

The important role of the thyroid axis in the body's response to various endogenous and exogenous factors is beyond doubt. The data on the effect of tumor growth on the functional activity of the thyroid gland are inconsistent. A modifying effect of chronic neurogenic pain on the biological aggressiveness of transplantable B16/F10 melanoma requires studying the main mechanisms of action. The aim of the study was to analyze the effect of chronic neurogenic pain on the functional activity of the thyroid gland in the dynamics of B16/F10 melanoma growth in male mice. Methods. The comparison group included animals with standard subcutaneous transplantation of B16/F10 melanoma; the main group - mice with B16/F10 melanoma transplanted 2 weeks after the generation of the chronic neurogenic pain model. Levels of total and free forms of thyroid hormones and TSH were determined in thyroid cytosolic fractions by standard ELISA methods. Results. Chronic neurogenic pain increases the levels of free forms of thyroid hormones and total triiodothyronine, but decreases the levels of TSH and total thyroxin in the thyroid, as well as the organ weight coefficient in male mice with transplanted B16/F10 melanoma. Conclusions. The results demonstrated that the thyroid activity was a point of application of modifying influence of chronic neurogenic pain on the course of transplantable B16/F10 melanoma in male mice.