Effect of cholinotropic agents to change the content of oxygen triplet forms of liver tissue and the ability of the liver homogenate to produce active oxygen in rat cooling

Abstract

The method of polarographic analysis of elements in the tissue solutions of living organisms, and the method of checking the ability of the liver homogenate to produce active forms of oxygen are among the methods for assessing the content of triplet forms of oxygen in the tissue and the ability of the tissue homogenate to initiate lipid peroxidation (POL) after a cold load and administration of cholinotropic agents to rats. In the study, the content of triplet form of oxygen of liver homogenate and the ability of the liver homogenate to produce active oxygen in the period of 3 hour and 5 days cooling of experimental animals were determined. The effect of cholinotropic agent accumulating the endogenous acetylcholine in liver tissue, pharmacological agents that stimulate and block the work of muscarine-sensitive cholinoreactive structures of hepatocyte plasmatic membranes with the assessment of their impact on the content of triplet forms of oxygen, the ability of the liver homogenate to produce active oxygen in supercooling of animals were investigated. Neostigmine on the background of 3 hours cold exposure led to a decrease in the content of triplet forms of oxygen but increased the ability of the liver homogenate to produce active forms of oxygen. Pilocarpine and atropine on the background of cooling animals in 5 days period caused the manifestation of reciprocity as in 3 minutes, so as in 30 minutes of experiment for the determination of triplet forms of oxygen of the liver tissue. Besides pilocarpine and atropine reduced the ability of liver tissue to produce active forms of oxygen. The obtained results indicate the change in the content of triplet forms of oxygen in the liver tissue when pilocarpine and atropine administrated to animals on the backdrop of 5 days cooling. Additionally the results show that the injection of neostigmine to animals on the background of 3 hours cooling promotes the increase of active forms of oxygen.