Effect of cholinesterase inhibitor galantamine on intestinal barrier function in rats with sepsis

Abstract

Objective To investigate the effects of galantamine treatment on intestinal function in rats with sepsis.Methods Healthy male Sprague-Dawley(SD) rats were randomly divided into five experimental groups:Sham CLP group:rats were exposure abdominal cavity only.CLP group:cecal ligation and puncture (CLP) method to establish sepsis model.CLP+galantamine group:adminisration galantamine (5 mg/kg.ip) 1 h after CLP.vagotomy+CLP+galantamine group;right vagotomy 3 d before CLP.Sham vagotomy+CLP+galantamine group:Sham right vagotomy 3 d before CLP.Observed the survival rate of each group for 72 h.Rats were sacrificed at 12 h after CLP to observe the histopathological change of intestinal,and obtained the blood sample to test the endotoxin level,collected homogenated tissues of mesenteric lymph nodes,liver and spleen to determine bacterial translocation.Results The survival rate of rats in CLP group was significantly lower than in Sham CLP group (20％ vs 100％),and the serum endotoxin level and bacterial translocation were higher in CLP group than in Sham CLP group (61.1％ vs 11.1％,P＜0.05),galantamine administration could obviously decrease endotoxin level and bacterial translocation (27.8％ vs 61.1％,P＜0.05),however,vagotomy before CLP operation could abolish the protect effects of galantamine (27.8％ vs 61.1％,P＜0.05).Conclusions Galantamine can reduce the degree of intestinal mucosa damage and bacterial translocation,increase the survival rate.Activation of vagus nerve might coutribute to these effect. Key words: Sepsis; Galantamine; Intestinal mucosa barrier; Bacterial translocation; Cholinergic anti-inflammation pathway