Effect of cholinergic antagonists on the bradycardic response of limpets to copper

Abstract

The effect of cholinergic antagonists on the bradycardia induced by waterborne copper in the Mediterranean limpet Patella caerulea was investigated by using non-invasive recording of cardiac activity of whole animals. Preliminary tests were conducted to check the role of cholinergic and serotoninergic systems in the control of heart rate of P. caerulea. Superfusing the whole limpets with carbachol (cholinergic agonist) at 5×10−5 M produced a negative inotropic and chronotropic effect (bradycardia), while superfusion with 5-hydroxytryptamine produced a positive inotropic and chronotropic effect (tachycardia). Exposure of limpets to a solution of copper in artificial seawater (0.25 mg l−1, 3 h) reduced their heart rate to about 80% the value recorded in copper-free water. This bradycardia was inhibited by injecting the limpets with atropine (cholinergic muscarinic antagonist) at 21 μg g−1 wet flesh weight and with benzoquinonium [cholinergic nicotinic antagonist blocking the K+ mediated acetylcholine (ACh) response] at 10 and 100 μg g−1 prior to copper exposure. In contrast, D-tubocurarine (cholinergic nicotinic antagonist blocking Na+ mediated ACh response) had no effect at 85 μg g−1. These results agree with the involvement of the cholinergic system in the bradycardic response of limpets to copper, and support the view that gastropod ACh receptors do not fit the vertebrate nicotinic–muscarinic classification.