Abstract
Deuterium nuclear magnetic resonance and differential scanning calorimetery were used to study the phase behavior, chain order, and dynamics of bilayers consisting of 1-palmitoyl-2-arachidonoyl phosphatidylcholine perdeuterated on the saturated acyl chain (16:0-20:4 PC-d31 ) alone and in mixed bilayers containing 15 and 30 mol % cholesterol. In the liquid crystalline phase, saturated chain orientational order increases with increasing cholesterol concentration. The transition to a highly ordered phase is centered on −25 °C regardless of cholesterol concentration. The observation of coexisting ordered and liquid crystalline phase spectral contributions near the transition in the absence of cholesterol implies a discontinuous transition. In the presence of cholesterol, the phase change is continuous and no evidence of coexisting domains of ordered and fluid phase lipid is observed. The observation of spectral intensity near ±63 kHz in the ordered phase suggests near-immobilization of the methylene deuterons...
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