Abstract
ObjectiveTo assess the effect of cholesterol loaded cyclodextrin on seminal attributes (progressive motility, livability, hypo-osmotic swelling (HOS) response and acrosomal integrity), lipid peroxidation (LPO) and reactive oxygen species (ROS) levels during cryopreservation of buffalo (Bubalus bubalis) spermatozoa. MethodsA total of 24 ejaculates (mass motility ≥4; progressive motility ≥80%) were collected from three murrah buffalo bulls (8 from each bull) through artificial vagina twice a week. One part of semen was diluted with TEYC extender (group I) and other part was treated with cholesterol loaded cyclodextrin before final dilution with TEYC extender (group II). Semen samples were evaluated for various seminal attributes, LPO and ROS levels at fresh, pre-freeze and post-thaw stage in group I and group II. ResultsSeminal attributes (progressive motility, livability, hypo-osmotic swelling (HOS) response and acrosomal integrity) were significantly higher in group II than group I at pre-freeze (P < 0.05) and post-thaw (P < 0.01) stage. LPO and ROS levels were significantly higher in group I as compared to group II at pre-freeze (P < 0.05) and post-thaw (P < 0.01) stage. ConclusionIt is concluded that cholesterol loaded cyclodextrin reduces LPO and ROS levels during cryopreservation of buffalo spermatozoa.
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