Abstract
In this work, we compared the effects of curcumin and cholesterol directly competing to insert into the DMPC lipid bilayer during bilayer formation from an initially non-ordered state. 2H and 14N nuclear magnetic resonance spectroscopy showed that curcumin is not embedded deep in the lipid bilayer and interacts mainly with the head group of the lipid. In a more complex system of DMPC/CHOL/CUR, curcumin amplifies the effect of cholesterol on the ordering of lipid acyl chains.
Highlights
Cholesterol (CHOL) is an essential component of mammalian cell membranes
We studied the combined effect of both CUR and CHOL on a lipid bilayer, using 2H and 14N nuclear magnetic resonance (NMR) as methods to study ordering of lipid membranes
Formation of vesicles in all studied DMPC/CHOL/CUR samples was confirmed by observation of resolved 2H NMR spectra. 31P NMR and 2H NMR experiments performed on vesicles of DMPC-d54 in the presence of CHOL and CUR
Summary
Cholesterol (CHOL) is an essential component of mammalian cell membranes. It is embedded in the hydrophobic part of the membrane with its hydroxyl group interacting with the polar head groups of the membrane phospholipids and sphingolipids [1, 2]. Solid-state NMR on magnetically aligned bicelles of DMPC and DSC on bilayers of DMPC and DPPC have shown CUR has a strong effect on membrane structure at low concentrations [22]. Like CHOL, CUR binds DMPC in a transbilayer orientation at low concentrations and induces segmental ordering in the lipid membrane [23]. Lateral diffusion on planar lipid multibilayers and ordering of lipids on multibilayered vesicles have been studied by 1H and 2H NMR of DOPC and DMPC at concentrations of CUR varied in the range of 0–40 mol% [24]. We studied the combined effect of both CUR and CHOL on a lipid bilayer, using 2H and 14N NMR as methods to study ordering of lipid membranes
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
