Effect of Chlorpyrifos on Antioxidant Enzyme Activities and Lipid Peroxidation in Liver and Gills of Zebrafish

Abstract

Chlorpyrifos (CP) is a widely used and studied organophosphorous pesticide for more than a decade to control pests in agriculture, including termites, mosquitoes, flies and other household pests. Because of the extensive use of CP, besides destroying the target pests also affecting the non-target organisms like human, fish and finally finding their way to the aquatic environment. Fish is not only a vital source of food for people but also an important component of ecosystem and is shown to be sensitive bioindicator of external factors, such as pollution. In the present investigative study zebrafish, (Danio rerio) an excellent model for toxicological studies was exposed to CP (200 μg/L) and the oxidative parameters like catalase (CAT), Superoxide Dismutase (SOD), Glutathione Reductase (GR), Glutathione Peroxidase (GPx), Glutathione-s-transferase (GST) and Lipid Peroxidation (LPO) in liver and gill tissues of zebrafish were assessed. The antioxidant enzyme parameters were significantly decreased in the treated groups than in control with concomitant increase in detoxifying processes i.e., GST and LPO. The results are discussed in detail.