Effect of chlorocholine chlorid on phenolic acids accumulation and polyphenols formation of buckwheat plants.

Oksana Sytar,Irene Hemmerich,Iryna Smetanska,Asel Borankulova,Cornelia Rauh

doi:10.1186/0717-6287-47-19

Abstract

BackgroundEffect of chlorocholine chloride (CCC) on phenolic acids composition and polyphenols accumulation in various anatomical parts (stems, leaves and inflorescences) of common buckwheat (Fagopyrum esculentum Moench) in the early stages of vegetation period were surveyed.ResultsTreatment of buckwheat seeds with 2% of CCC has been increased content of total phenolics in the stems, leaves and inflorescences. On analyzing the different parts of buckwheat plants, 9 different phenolic acids – vanilic acid, ferulic acid, trans-ferulic acid, chlorogenic acid, salycilic acid, cinamic acid, p-coumaric acid, p-anisic acid, methoxycinamic acid and catechins were identified. The levels of identified phenolic acids varied not only significantly among the plant organs but also between early stages of vegetation period. Same changes as in contents of chlorogenic acid, ferulic acid, trans-ferulic acid were found for content of salycilic acid. The content of these phenolic acids has been significant increased under effect of 2% CCC treatment at the phase I (formation of buds) in the stems and at the phase II (beginning of flowering) in the leaves and then inflorescences respectively. The content of catechins as potential buckwheat antioxidants has been increased at the early stages of vegetation period after treatment with 2% CCC.ConclusionsThe obtained results suggest that influence of CCC on the phenolics composition can be a result of various mechanisms of CCC uptake, transforming and/or its translocation in the buckwheat seedlings.

Highlights

Effect of chlorocholine chloride (CCC) on phenolic acids composition and polyphenols accumulation in various anatomical parts of common buckwheat (Fagopyrum esculentum Moench) in the early stages of vegetation period were surveyed
In the variants with CCC treatment of buckwheat plants at phase I it was visible tendency of increasing content of total phenolics in the leaves on 9% compared to the control, Figure 1
Content of total phenolics in the leaves of buckwheat plants has been found tendency of increasing on 8% compared to the control

Summary

Introduction

Effect of chlorocholine chloride (CCC) on phenolic acids composition and polyphenols accumulation in various anatomical parts (stems, leaves and inflorescences) of common buckwheat (Fagopyrum esculentum Moench) in the early stages of vegetation period were surveyed. Buckwheat achene contain mostly carbohydrate, especially starch, 55,8% [1]. The starch content in buckwheat grains is 55,8%, in bran 40,7% and in the flour 78,4%. The protein content 11,7% in buckwheat grains, in bran 21,6% and in the flour 10,6% [1] Buckwheat protein content for different buckwheat species near 11-15% which is similar to the protein content in cereal grains. In cereals, 10-20% of the protein lies in the embryo, while 80-90% is found in the endosperm. In buckwheat 55% of the protein is located in embryo, 35% in the endosperm, and the reminder found in the hull [2].

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Conclusion