Abstract
In bacteriophage φX174 infection, the net synthesis of replicative form DNA ceased between 15 and 20min after infection. When 30μg of chloramphenicol/ml was added, net RF synthesis, however, continued beyond the normal time and level of turn-off. Ex-periments with φX174 mutants unable to synthesize single-stranded DNA showed that a protein synthesis was required for the cessation of net RF synthesis and the protein was synthesized between 10 and 15 min after infection.
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