Effect of chitosan oligosaccharide and mycotoxin adsorbents on intestinal mucosa structure and gut flora of Litopenaeus vannamei

Abstract

PDF HTML阅读 XML下载 导出引用 引用提醒 饲料中添加壳寡糖和霉菌毒素吸附剂对凡纳滨对虾肠道黏膜形态及菌群结构的影响 DOI: 作者: 作者单位: 广东海洋大学 水产动物营养与饲料实验室, 广东 湛江 524088 作者简介: 黄钦成(1993-),男,硕士研究生,研究方向为水产动物营养与饲料.E-mail:qinchengh0814@163.com 通讯作者: 中图分类号: S963 基金项目: 渔港建设和渔业产业发展专项（A201608C06）；湛江市科技计划项目（2016A03022）；广东海洋大学人才培训财政专项（002024008）. Effect of chitosan oligosaccharide and mycotoxin adsorbents on intestinal mucosa structure and gut flora of Litopenaeus vannamei Author: Affiliation: Laboratory of Aquatic Animal Nutrition and Feed, Guangdong Ocean University, Zhanjiang 524088, China Fund Project: 摘要 | 图/表 | 访问统计 | 参考文献 | 相似文献 | 引证文献 | 资源附件 | 文章评论 摘要:实验旨在研究在饲料中分别或联合添加壳寡糖（chitosan oligosaccharide，COS）和霉菌毒素吸附剂（mycotoxins adsorbent）对凡纳滨对虾（）肠道黏膜形态及菌群结构的影响。在基础饲料（对照组）中分别添加250 mg/kg COS、2500 mg/kg霉菌毒素吸附剂、250 mg COS+2500 mg/kg霉菌毒素吸附剂（组号为C0、C0.25、M2.5、C0.25+M2.5），制成4组等氮等能饲料，投喂初重（0.23±0.02）g的凡纳滨对虾8周。结果显示：COS与霉菌毒素吸附剂联合使用有助于改善肠道形态学指标，C0.25+M2.5组绒毛高显著大于C0、C0.25组（<0.05）。高通量测序结果表明各组有效操作分类单元（Operational Taxonomic Unit，OTUs）数目无显著性差异（<0.05），但显著高于C0.25组（>0.05）；肠道菌群主要为变形菌门（Proteobacteria）、厚壁菌门（Firmicutes）和拟杆菌门（Bacteroidetes），C0.25+M2.5组变形菌门细菌含量最低且厚壁菌门细菌含量最高，各组拟杆菌门细菌均升高，C0组含量最低。在属水平上，弧菌属（Photobacterium）以及占主要优势，C0.25+M2.5组弧菌属、发光杆菌属细菌含量均有效减少；C0.25+M2.5组发光杆菌属细菌含量最低，假交替单胞菌属细菌含量最高。250 mg/kg COS和2500 mg/kg霉菌毒素吸附剂联合添加对菌群丰富度无显著影响，但优化了肠道形态学指标及肠道菌群结构，提升了肠道健康，效果优于单一添加组。 Abstract:A 56-day feed trial was carried out to investigate the effects of chitosan oligosaccharide (COS) and/or mycotoxin adsorbent on the intestinal mucosa structure and gut flora of . Four isonitrogenous and homoenergetic diets were prepared on the basal diet by adding 0 mg/kg (COS or mycotoxin adsorbent), 250 mg/kg COS, 2500 mg/kg mycotoxin adsorbent, 250 mg/kg COS+2500 mg/kg mycotoxin adsorbent, and named as C0, C0.25, M2.5 and C0.25+M2.5, respectively. The with an initial weight of 0.23±0.02 g were randomly assigned into 4 groups. The results showed as follows:the combined use of COS and mycotoxin adsorbent helped improve the index of intestinal morphology, group C0.25+M2.5 showed significant difference (<0.05) about the intestinal villus length with group C0, C0.25, and showed significant difference (<0.05) about the intestinal width with group M2.5, moreover, the thickness of muscle layer in this group was significantly higher than others (>0.05) about effective OTUs (Operational taxonomic unit) among groups; index of Observed species and Shannon index and PD (Phylogenetic diversity whole tree) value in group C0.25+M2.5 were significantly lower (<0.05) than those of group M2.5 and remarkably higher than group C0.25, but showed no significant difference (>0.05) with group C0. At the phylum level, microbial floras detected in this experiment mainly belonged to Proteobacteria, Firmicutes or Bacteroidetes, group C0.25+M2.5 showed the minimum of Proteobacteria and the maximum of Firmicutes, group C0 showed the minimum of Bacteroidetes. At the level of genus, species mainly detected were and in group C0.25+M2.5 were lower than that of control group. Group C0.25+M2.5 showed the lowest content of Photobacterium and the highest content of Pseudoalteromonas. It can be concluded that adding COS and mycotoxin adsorbent in diets had no effect of on microbial floras, nevertheless, optimized the index of intestinal morphology and the structure of gut flora, but can promoted intestinal health, and is better than a single adding groups. 参考文献 相似文献 引证文献