Abstract
(1) Background: The effectiveness of chitosan to improve the action of antimicrobial compounds against planktonic bacteria and young biofilms has been widely investigated in Dentistry, where the biofilm lifecycle is a determining factor for the success of antibacterial treatment. In the present study, mature Streptococcus mutans biofilms were treated with chitosan dispersion (CD) or chitosan microparticles (CM). (2) Methods: CD at 0.25% and 1% were characterized by texture analysis, while CD at 2% was spray-dried to form CM, which were characterized with respect to particle size distribution, zeta potential, and morphology. After determining the minimum inhibitory and bactericidal concentrations, S. mutans biofilms were grown on glass slides exposed 8×/day to 10% sucrose and 2×/day to CD or CM at 0.25% and 1%. Biofilm viability and acidogenicity were determined, using appropriate control groups for each experiment. (3) Results: CD had high viscosity and CM were spherical, with narrow size distribution and positive zeta potential. CM affected bacterial viability and acidogenicity in mature S. mutans biofilms more strongly than CD, especially at 1%. (4) Conclusions: Both chitosan forms exerted antimicrobial effect against mature S. mutans biofilms. CM at 1% can reduce bacterial viability and acidogenicity more effectively than CD at 1%, and thereby be more effective to control the growth of mature biofilms in vitro.
Highlights
The use of natural antimicrobial products for the control and prevention of several oral diseases has been extensively studied [1]
Several approaches have been reported to improve the antimicrobial activity of this natural polysaccharide, including formation of micro- and nanoparticles [4]
[16], and the importance of this highly structured polysaccharide matrix of antimicrobial agents, importance of thispotential highly structured polysaccharide matrixexamined for diffusion of antimicrobial agents, and the antimicrobial of chitosan, the present study the physicochemical properties thetwo antimicrobial potential of chitosan, the present study examined the physicochemical properties of dosage forms of chitosan dispersion and microparticles, and their antibacterial effect against of two dosage of chitosanmature dispersion and of microparticles, planktonic formforms and structured biofilms
Summary
The use of natural antimicrobial products for the control and prevention of several oral diseases has been extensively studied [1]. A natural polysaccharide of marine origin, has attracted attention in the Dentistry area due to its significant biological properties, biodegradability, biocompatibility, lack of toxicity [2], and effectiveness against microorganisms that cause oral diseases, such as dental caries [1]. This polysaccharide exhibits stronger antibacterial activity under acidic conditions (pH < 6.5) [1], at which it forms a dispersion due to protonation of free amino groups that causes electrostatic repulsion and polymer solvation, and mediates its antimicrobial activity [3].
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
