Effect of chitosan coating in the shelf life and control of Listeria monocytogenes growth on the surface of ready-to-eat bovine meatballs during chilled storage

Abstract

Edible chitosan coating on the surface of ready-to-eat (RTE) bovine meatballs was evaluated for its effect in the shelf life of them and the control of Listeria monocytogenes at 5 °C. L. monocytogenes was inoculated onto the surface of RTE bovine meatballs with and without edible chitosan coating. The samples were stored at 5 °C. Total aerobic viable count (TVC) and the bacterial counts of L. monocytogenes , lactic acid bacteria and Enterobacteriaceae were determined on days 0,1,7,14,21 and 28. The sensory characteristics were also evaluated at the same time spots by semi trained panelists. The analysis depicted that the use of edible chitosan membranes reduced all of the microbial populations that were enumerated, and retard their growth leading to the conclusion that they can prolong the span life of these products by 14 days. Moreover, the population of the inoculated L. monocytogenes was about 2 log CFU/g lower in the meatballs coated with chitosan, indicating an inhibitory effect of chitosan in the growth of L. monocytogenes . The sensory analysis showed that the coated with chitosan samples were satisfactorily accepted by the panelists even at day 28, in contrast to the samples without chitosan (control samples) which were unacceptable at day 14. These results indicate that edible chitosan coatings represent a potential agent in controlling L. monocytogenes on the surface of RTE meatballs as well as other RTE meat products, prolonging their shelf life without affecting their sensory characteristics.